This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: ALDH18A1, Human
PrimePCR™ Template for SYBR® Green Assay: ALDH18A1, Human
This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate a critical step in the de novo biosynthesis of proline ornithine and arginine. Mutations in this gene lead to hyperammonemia hypoornithinemia hypocitrullinemia hypoargininemia and hypoprolinemia and may be associated with neurodegeneration cataracts and connective tissue diseases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.