This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage
PrimePCR™ PreAmp for SYBR® Green Assay: SEMA4G, Human
PrimePCR™ Template for SYBR® Green Assay: SEMA4G, Human
Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities semaphorins are put into eight classes: invertebrates contain classes 1 and 2 viruses have class V and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.