PrimePCR™ SYBR® Green Assay: PPP1R12B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018241
Assay Design:   Intron-spanning
Chromosome Location:   1:202531987-202533601question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000406302 ENST00000336894 ENST00000391959 ENST00000367270

Gene Information

Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta protein phosphatase 1 catalytic subunit delta) a large regulatory subunit (MYPT myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated--MYPT1 and MYPT2 the first of which is widely expressed and the second of which may be specific to heart skeletal muscle and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. This locus encodes both MYTP2 and M20. Alternatively spliced transcript variants encoding different isoforms have been identified. Related pseudogenes have been defined on the Y chromosome. [provided by RefSeq Oct 2011]

Gene Symbol:   PPP1R12B
Gene Name:   protein phosphatase 1, regulatory (inhibitor) subunit 12B
Aliases:   FLJ40942, MGC131980, MGC87886, MYPT2
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000077157
Entrez:   4660
UniGene:   Hs.444403
Chromosome Mapping:   1q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.390000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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