This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: CLCA2, Human
PrimePCR™ Template for SYBR® Green Assay: CLCA2, Human
The protein encoded by this gene belongs to the calcium sensitive chloride conductance protein family. To date all members of this gene family map to the same site on chromosome 1p31-p22 and share high degrees of homology in size sequence and predicted structure but differ significantly in their tissue distributions. Since this protein is expressed predominantly in trachea and lung it is suggested to play a role in the complex pathogenesis of cystic fibrosis. It may also serve as adhesion molecule for lung metastatic cancer cells mediating vascular arrest and colonization and furthermore it has been implicated to act as a tumor suppressor gene for breast cancer. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.