PrimePCR™ SYBR® Green Assay: CLCA2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018184
Assay Design:   Intron-spanning
Chromosome Location:   1:86905977-86907223question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000370565

Gene Information

The protein encoded by this gene belongs to the calcium sensitive chloride conductance protein family. To date all members of this gene family map to the same site on chromosome 1p31-p22 and share high degrees of homology in size sequence and predicted structure but differ significantly in their tissue distributions. Since this protein is expressed predominantly in trachea and lung it is suggested to play a role in the complex pathogenesis of cystic fibrosis. It may also serve as adhesion molecule for lung metastatic cancer cells mediating vascular arrest and colonization and furthermore it has been implicated to act as a tumor suppressor gene for breast cancer. [provided by RefSeq Jul 2008]

Gene Symbol:   CLCA2
Gene Name:   chloride channel accessory 2
Aliases:   CACC, CACC3, CLCRG2, CaCC-3, FLJ97885
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000137975
Entrez:   9635
UniGene:   Hs.241551
Chromosome Mapping:   1p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.450000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download