This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: FIG4, Human
PrimePCR™ Template for SYBR® Green Assay: FIG4, Human
The protein encoded by this gene belongs to the SAC domain-containing protein gene family. The SAC domain approximately 400 amino acids in length and consisting of seven conserved motifs has been shown to possess phosphoinositide phosphatase activity. The yeast homolog Sac1p is involved in the regulation of various phosphoinositides and affects diverse cellular functions such as actin cytoskeleton organization Golgi function and maintenance of vacuole morphology. Membrane-bound phosphoinositides function as signaling molecules and play a key role in vesicle trafficking in eukaryotic cells. Mutations in this gene have been associated with Charcot-Marie-Tooth disease type 4J. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.