PrimePCR™ SYBR® Green Assay: PIWIL4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031619

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PIWIL4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PIWIL4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018049
Assay Design:   Intron-spanning
Chromosome Location:   11:94310558-94316650question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000299001 ENST00000545603

Gene Information

PIWIL4 belongs to the Argonaute family of proteins which function in development and maintenance of germline stem cells (Sasaki et al. 2003 [PubMed 12906857]).[supplied by OMIM Mar 2008]

Gene Symbol:   PIWIL4
Gene Name:   piwi-like 4 (Drosophila)
Aliases:   DKFZp686P01248, FLJ36156, HIWI2, MIWI2
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000134627
Entrez:   143689
Chromosome Mapping:   11q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.870000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download