PrimePCR™ SYBR® Green Assay: PIWIL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031498

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PIWIL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PIWIL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017852
Assay Design:   Intron-spanning
Chromosome Location:   12:130831547-130833816question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000245255 ENST00000540672

Gene Information

This gene encodes a member of the PIWI subfamily of Argonaute proteins evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal RNA silencing and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2010]

Gene Symbol:   PIWIL1
Gene Name:   piwi-like 1 (Drosophila)
Aliases:   HIWI, MIWI, PIWI
RefSeq:   NC_000012.11 NT_009755.19
Ensembl:   ENSG00000125207
Entrez:   9271
Chromosome Mapping:   12q24.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.991000
y-intercept 33.330000
Efficiency 110

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download