This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0031455
PrimePCR™ PreAmp for SYBR® Green Assay: NUDT12, Human
PrimePCR™ Template for SYBR® Green Assay: NUDT12, Human
Nucleotides are involved in numerous biochemical reactions and pathways within the cell as substrates cofactors and effectors. Nudix hydrolases such as NUDT12 regulate the concentrations of individual nucleotides and of nucleotide ratios in response to changing circumstances (Abdelraheim et al. 2003 [PubMed 12790796]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.