This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0031414
PrimePCR™ PreAmp for SYBR® Green Assay: KDM4C, Human
PrimePCR™ Template for SYBR® Green Assay: KDM4C, Human
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with one JmjC domain one JmjN domain two PHD-type zinc fingers and two Tudor domains. This nuclear protein functions as a trimethylation-specific demethylase converting specific trimethylated histone residues to the dimethylated form. Chromosomal aberrations and increased transcriptional expression of this gene are associated with esophageal squamous cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq Apr 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.