PrimePCR™ SYBR® Green Assay: MYOF, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017665
Assay Design:   Intron-spanning
Chromosome Location:   10:95185877-95191225question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000358334 ENST00000359263 ENST00000371489 ENST00000371488 ENST00000371501 ENST00000371502

Gene Information

Mutations in dysferlin a protein associated with the plasma membrane can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected but their full-length nature has not been determined. [provided by RefSeq Dec 2008]

Gene Symbol:   MYOF
Gene Name:   myoferlin
Aliases:   FER1L3, FLJ36571, FLJ90777
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000138119
Entrez:   26509
UniGene:   Hs.602086
Chromosome Mapping:   10q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.570000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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