This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0031245
PrimePCR™ PreAmp for SYBR® Green Assay: AGTR2, Human
PrimePCR™ Template for SYBR® Green Assay: AGTR2, Human
The protein encoded by this gene belongs to the G-protein coupled receptor 1 family and functions as a receptor for angiotensin II. It is an intergral membrane protein that is highly expressed in fetus but scantily in adult tissues except brain adrenal medulla and atretic ovary. This receptor has been shown to mediate programmed cell death and this apoptotic function may play an important role in developmental biology and pathophysiology. Mutations in this gene are been associated with X-linked mental retardation. [provided by RefSeq Jan 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.