This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0031239
PrimePCR™ PreAmp for SYBR® Green Assay: WFDC1, Human
PrimePCR™ Template for SYBR® Green Assay: WFDC1, Human
This gene encodes a member of the WAP-type four disulfide core domain family. The WAP-type four-disulfide core domain or WAP signature motif contains eight cysteines forming four disulfide bonds at the core of the protein and functions as a protease inhibitor in many family members. The encoded protein shares 81% amino acid identity with the rat ps20 protein which was originally identified as a secreted growth inhibitor. This gene is mapped to chromosome 16q24 an area of frequent loss of heterozygosity in cancers including prostate breast and hepatocellular cancers and Wilms' tumor. Owing to its location and a possible growth inhibitory property of its gene product this gene is suggested to be a tumor suppressor gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.