PrimePCR™ SYBR® Green Assay: WFDC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031239

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: WFDC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: WFDC1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017436
Assay Design:   Intron-spanning
Chromosome Location:   16:84358046-84362979question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000219454

Gene Information

This gene encodes a member of the WAP-type four disulfide core domain family. The WAP-type four-disulfide core domain or WAP signature motif contains eight cysteines forming four disulfide bonds at the core of the protein and functions as a protease inhibitor in many family members. The encoded protein shares 81% amino acid identity with the rat ps20 protein which was originally identified as a secreted growth inhibitor. This gene is mapped to chromosome 16q24 an area of frequent loss of heterozygosity in cancers including prostate breast and hepatocellular cancers and Wilms' tumor. Owing to its location and a possible growth inhibitory property of its gene product this gene is suggested to be a tumor suppressor gene. [provided by RefSeq Jul 2008]

Gene Symbol:   WFDC1
Gene Name:   WAP four-disulfide core domain 1
Aliases:   PS20
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000103175
Entrez:   58189
Chromosome Mapping:   16q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.120000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download