PrimePCR™ SYBR® Green Assay: WFDC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031239

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017436
Assay Design:   Intron-spanning
Chromosome Location:   16:84358046-84362979question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000219454

Gene Information

This gene encodes a member of the WAP-type four disulfide core domain family. The WAP-type four-disulfide core domain or WAP signature motif contains eight cysteines forming four disulfide bonds at the core of the protein and functions as a protease inhibitor in many family members. The encoded protein shares 81% amino acid identity with the rat ps20 protein which was originally identified as a secreted growth inhibitor. This gene is mapped to chromosome 16q24 an area of frequent loss of heterozygosity in cancers including prostate breast and hepatocellular cancers and Wilms' tumor. Owing to its location and a possible growth inhibitory property of its gene product this gene is suggested to be a tumor suppressor gene. [provided by RefSeq Jul 2008]

Gene Symbol:   WFDC1
Gene Name:   WAP four-disulfide core domain 1
Aliases:   PS20
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000103175
Entrez:   58189
UniGene:   Hs.36688
Chromosome Mapping:   16q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.120000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download