This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0031206
PrimePCR™ PreAmp for SYBR® Green Assay: SNRPB, Human
PrimePCR™ Template for SYBR® Green Assay: SNRPB, Human
The protein encoded by this gene is one of several nuclear proteins that are found in common among U1 U2 U4/U6 and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.