PrimePCR™ SYBR® Green Assay: NR1H2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031117

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017248
Assay Design:   Intron-spanning
Chromosome Location:   19:50883045-50885275question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000253727 ENST00000411902 ENST00000376942 ENST00000542413

Gene Information

The liver X receptors LXRA (NR1H3; MIM 602423) and LXRB form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver adrenal gland intestine adipose tissue macrophages lung and kidney whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al. 2009 [PubMed 19436111]).[supplied by OMIM Jan 2010]

Gene Symbol:   NR1H2
Gene Name:   nuclear receptor subfamily 1, group H, member 2
Aliases:   LXR-b, LXRB, NER, NER-I, RIP15, UNR
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000131408
Entrez:   7376
UniGene:   Hs.432976
Chromosome Mapping:   19q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.960000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download