This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: TNK1, Human
PrimePCR™ Template for SYBR® Green Assay: TNK1, Human
The protein encoded by this gene belongs to the tyrosine protein kinase family. Tyrosine protein kinases are important regulators of intracellular signal transduction pathways mediating cellular proliferation survival and development. This gene is highly expressed in fetal tissues and at lower levels in few adult tissues thus may function in signaling pathways utilized broadly during fetal development and more selectively in adult tissues. It plays a negative regulatory role in the Ras-Raf1-MAPK pathway and knockout mice have been shown to develop spontaneous tumors suggesting a role as a tumor suppressor gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.