PrimePCR™ SYBR® Green Assay: TRPM4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: TRPM4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TRPM4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017057
Assay Design:   Intron-spanning
Chromosome Location:   19:49705340-49713586question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000252826 ENST00000427978 ENST00000355712

Gene Information

The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration but this channel does not transport calcium. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2010]

Gene Symbol:   TRPM4
Gene Name:   transient receptor potential cation channel, subfamily M, member 4
Aliases:   FLJ20041, PFHB1B, TRPM4B
RefSeq:   NC_000019.9 NG_027551.1 NT_011109.16
Ensembl:   ENSG00000130529
Entrez:   54795
Chromosome Mapping:   19q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.960000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download