PrimePCR™ SYBR® Green Assay: RSPH4A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030981

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RSPH4A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RSPH4A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017043
Assay Design:   Intron-spanning
Chromosome Location:   6:116938394-116944003question
Amplicon Length:   122
Splice Variants Targeted:   ENST00000229554 ENST00000368580 ENST00000368581 ENST00000447842

Gene Information

This gene encodes a protein that appears to be a component the radial spoke head as determined by homology to similar proteins in the biflagellate alga Chlamydomonas reinhardtii and other ciliates. Radial spokes which are regularly spaced along cilia sperm and flagella axonemes consist of a thin 'stalk' and a bulbous 'head' that form a signal transduction scaffold between the central pair of microtubules and dynein. Mutations in this gene cause primary ciliary dyskinesia 1 a disease arising from dysmotility of motile cilia and sperm. Alternative splicing results in multiple transcript variants. [provided by RefSeq Sep 2009]

Gene Symbol:   RSPH4A
Gene Name:   radial spoke head 4 homolog A (Chlamydomonas)
Aliases:   CILD11, FLJ37974, MGC126303, RSHL3, RSPH6B, dJ412I7.1
RefSeq:   NC_000006.11 NG_012934.1 NT_025741.15
Ensembl:   ENSG00000111834
Entrez:   345895
Chromosome Mapping:   6q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 34.620000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download