This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: HSD17B14, Human
PrimePCR™ Template for SYBR® Green Assay: HSD17B14, Human
17-beta-hydroxysteroid dehydrogenases such as HSD17B14 are primarily involved in metabolism of steroids at the C17 position and also of other substrates such as fatty acids prostaglandins and xenobiotics (Lukacik et al. 2007 [PubMed 17067289]).[supplied by OMIM Jun 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.