This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030842
PrimePCR™ PreAmp for SYBR® Green Assay: AP3D1, Human
PrimePCR™ Template for SYBR® Green Assay: AP3D1, Human
The protein encoded by this gene is a subunit of the AP3 adaptor-like complex which is not clathrin-associated but is associated with the golgi region as well as more peripheral structures. The AP-3 complex facilitates the budding of vesicles from the golgi membrane and may be directly involved in trafficking to lysosomes. This subunit is implicated in intracellular biogenesis and trafficking of pigment granules and possibly platelet dense granules and neurotransmitter vesicles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.