This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030784
PrimePCR™ PreAmp for SYBR® Green Assay: GLRX2, Human
PrimePCR™ Template for SYBR® Green Assay: GLRX2, Human
The protein encoded by this gene is a member of the glutaredoxin family of proteins which maintain cellular thiol homeostasis. These proteins are thiol-disulfide oxidoreductases that use a glutathione-binding site and one or two active cysteines in their active site. This gene undergoes alternative splicing to produce multiple isoforms one of which is ubiquitously expressed and localizes to mitochondria where it functions in mitochondrial redox homeostasis and is important for the protection against and recovery from oxidative stress. Other isoforms which have more restrictive expression patterns show cytosolic and nuclear localization and are thought to function in cellular differentiation and transformation possibly with a role in tumor progression. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.