PrimePCR™ SYBR® Green Assay: RRAGD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016675
Assay Design:   Intron-spanning
Chromosome Location:   6:90087427-90088981question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000359203 ENST00000369415

Gene Information

RRAGD is a monomeric guanine nucleotide-binding protein or G protein. By binding GTP or GDP small G proteins act as molecular switches in numerous cell processes and signaling pathways.[supplied by OMIM Apr 2004]

Gene Symbol:   RRAGD
Gene Name:   Ras-related GTP binding D
Aliases:   DKFZp761H171, FLJ44176, RAGD, bA11D8.2.1
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000025039
Entrez:   58528
UniGene:   Hs.31712
Chromosome Mapping:   6q15-q16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 34.630000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download