PrimePCR™ SYBR® Green Assay: SLC22A24, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030700

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC22A24, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC22A24, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016614
Assay Design:   Intron-spanning
Chromosome Location:   11:62886695-62902200question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000417740 ENST00000531535 ENST00000326192

Gene Information

SLC22A24 belongs to a large family of transmembrane proteins that function as uniporters symporters and antiporters to transport organic ions across cell membranes (Jacobsson et al. 2007 [PubMed 17714910]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC22A24
Gene Name:   solute carrier family 22, member 24
Aliases:   MGC34821, NET46
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000197658
Entrez:   283238
Chromosome Mapping:   11q12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.720000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download