This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030661
PrimePCR™ PreAmp for SYBR® Green Assay: CNPY1, Human
PrimePCR™ Template for SYBR® Green Assay: CNPY1, Human
Cnpy1 is expressed in the midbrain-hindbrain (MHB) boundary in zebrafish binds FGFR1 (MIM 136350) and plays a role in FGF signaling (Hirate and Okamoto 2006 [PubMed 16488878]).[supplied by OMIM Dec 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.