PrimePCR™ SYBR® Green Assay: WFDC6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030595

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: WFDC6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: WFDC6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016447
Assay Design:   Intron-spanning
Chromosome Location:   20:44166689-44168039question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000372670 ENST00000372665

Gene Information

This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. The WFDC domain or WAP signature motif contains eight cysteines forming four disulfide bonds at the core of the protein and functions as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the telomeric cluster. Read-through transcription exists between this gene and the upstream SPINLW1 (serine peptidase inhibitor-like with Kunitz and WAP domains 1) gene. [provided by RefSeq Nov 2010]

Gene Symbol:   WFDC6
Gene Name:   WAP four-disulfide core domain 6
Aliases:   C20orf171, MGC126649, MGC126653, WAP6, dJ461P17.11
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000243543
Entrez:   140870
Chromosome Mapping:   20q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.490000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download