This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030588
PrimePCR™ PreAmp for SYBR® Green Assay: TOMM34, Human
PrimePCR™ Template for SYBR® Green Assay: TOMM34, Human
The protein encoded by this gene is involved in the import of precursor proteins into mitochondria. The encoded protein has a chaperone-like activity binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane has a weak ATPase activity and contains 6 TPR repeats. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.