PrimePCR™ SYBR® Green Assay: VNN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030582

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: VNN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: VNN1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016426
Assay Design:   Intron-spanning
Chromosome Location:   6:133004399-133005547question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000367928

Gene Information

This gene encodes a member of the vanin family of proteins which share extensive sequence similarity with each other and also with biotinidase. The family includes secreted and membrane-associated proteins a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins however they possess pantetheinase activity which may play a role in oxidative-stress response. This protein like its mouse homolog is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to and in the same transcriptional orientation as two other vanin genes on chromosome 6q23-q24. [provided by RefSeq Feb 2009]

Gene Symbol:   VNN1
Gene Name:   vanin 1
Aliases:   HDLCQ8, MGC116930, MGC116931, MGC116932, MGC116933, Tiff66
RefSeq:   NC_000006.11 NG_012147.1 NT_025741.15
Ensembl:   ENSG00000112299
Entrez:   8876
Chromosome Mapping:   6q23-q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.580000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download