PrimePCR™ SYBR® Green Assay: HELZ, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030529

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HELZ, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HELZ, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016343
Assay Design:   Intron-spanning
Chromosome Location:   17:65214874-65239916question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000358691 ENST00000417253

Gene Information

HELZ is a member of the superfamily I class of RNA helicases. RNA helicases alter the conformation of RNA by unwinding double-stranded regions thereby altering the biologic activity of the RNA molecule and regulating access to other proteins (Wagner et al. 1999 [PubMed 10471385]).[supplied by OMIM Mar 2008]

Gene Symbol:   HELZ
Gene Name:   helicase with zinc finger
Aliases:   DHRC, DKFZp586G1924, DRHC, HUMORF5, KIAA0054, MGC163454
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000198265
Entrez:   9931
Chromosome Mapping:   17q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.070000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download