PrimePCR™ SYBR® Green Assay: SLC5A7, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016207
Assay Design:   Intron-spanning
Chromosome Location:   2:108625068-108626726question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000409059 ENST00000264047 ENST00000540517

Gene Information

Choline is a direct precursor of acetylcholine (ACh) a neurotransmitter of the central and peripheral nervous system that regulates a variety of autonomic cognitive and motor functions. SLC5A7 is a Na(+)- and Cl(-)- dependent high-affinity transporter that mediates the uptake of choline for acetylcholine synthesis in cholinergic neurons (Apparsundaram et al. 2000 [PubMed 11027560]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC5A7
Gene Name:   solute carrier family 5 (choline transporter), member 7
Aliases:   CHT, CHT1, MGC126299, MGC126300, hCHT
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000115665
Entrez:   60482
UniGene:   Hs.287758
Chromosome Mapping:   2q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.580000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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