PrimePCR™ SYBR® Green Assay: PFKFB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030423

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: PFKFB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PFKFB1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016187
Assay Design:   Intron-spanning
Chromosome Location:   X:54960268-54964043question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000375006 ENST00000545676 ENST00000374992

Gene Information

This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-26-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-26-biphosphate using independent catalytic domains. Fructose-26-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently regulating fructose-26-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. [provided by RefSeq Jul 2008]

Gene Symbol:   PFKFB1
Gene Name:   6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Aliases:   F6PK, HL2K, MGC116715, MGC116717, PFRX
RefSeq:   NC_000023.10 NG_012806.1 NG_013254.1 NT_011630.14
Ensembl:   ENSG00000158571
Entrez:   5207
Chromosome Mapping:   Xp11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.790000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download