This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030368
PrimePCR™ PreAmp for SYBR® Green Assay: CA10, Human
PrimePCR™ Template for SYBR® Green Assay: CA10, Human
This gene encodes a protein that belongs to the carbonic anhydrase family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes. The protein encoded by this gene is an acatalytic member of the alpha-carbonic anhydrase subgroup and it is thought to play a role in the central nervous system especially in brain development. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.