PrimePCR™ SYBR® Green Assay: SLC7A11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC7A11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC7A11, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016078
Assay Design:   Intron-spanning
Chromosome Location:   4:139093116-139100457question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000280612

Gene Information

This gene encodes a member of a heteromeric sodium-independent anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system designated Xc(-) the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels resulting in neuronal cell death. [provided by RefSeq Sep 2011]

Gene Symbol:   SLC7A11
Gene Name:   solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
Aliases:   CCBR1, xCT
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000151012
Entrez:   23657
Chromosome Mapping:   4q28-q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.960000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download