This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030328
PrimePCR™ PreAmp for SYBR® Green Assay: PHIP, Human
PrimePCR™ Template for SYBR® Green Assay: PHIP, Human
PHIP binds the pleckstrin homology (PH) domain of insulin receptor substrate-1 (IRS1; MIM 147545) modulates insulin signaling and plays a role in pancreatic beta cell growth and survival (Farhang-Fallah et al. 2000 [PubMed 11018022]; Podcheko et al. 2007 [PubMed 17636024]).[supplied by OMIM Jun 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.