PrimePCR™ SYBR® Green Assay: CNTNAP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: CNTNAP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CNTNAP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016009
Assay Design:   Intron-spanning
Chromosome Location:   17:40845474-40847591question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000264638

Gene Information

The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. The 1384-amino acid protein also designated p190 or CASPR for 'contactin-associated protein' includes an extracellular domain with several putative protein-protein interaction domains a putative transmembrane domain and a 74-amino acid cytoplasmic domain. Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb with weak expression in several other tissues tested. The architecture of its extracellular domain is similar to that of neurexins and this protein may be the signaling subunit of contactin enabling recruitment and activation of intracellular signaling pathways in neurons. [provided by RefSeq Jan 2009]

Gene Symbol:   CNTNAP1
Gene Name:   contactin associated protein 1
Aliases:   CASPR, CNTNAP, NRXN4, P190
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000108797
Entrez:   8506
Chromosome Mapping:   17q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997100
y-intercept 36.020000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download