This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: NPY, Human
PrimePCR™ Template for SYBR® Green Assay: NPY, Human
This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes including cortical excitability stress response food intake circadian rhythms and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase activate mitogen-activated protein kinase (MAPK) regulate intracellular calcium levels and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels higher alcohol consumption and may be a risk factor for various metabolic and cardiovascular diseases. [provided by RefSeq Nov 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.