This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030296
PrimePCR™ PreAmp for SYBR® Green Assay: RBP3, Human
PrimePCR™ Template for SYBR® Green Assay: RBP3, Human
Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity suggesting a series of gene duplication events. In the gene the boundaries of these domains are not defined by exon-intron junctions as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon which is 3180 base pairs long. The remainder of the fourth domain is encoded in the last three exons which are 191 143 and approximately 740 base pairs long respectively. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.