PrimePCR™ SYBR® Green Assay: PI15, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030250

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015904
Assay Design:   Intron-spanning
Chromosome Location:   8:75737730-75756304question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000523773 ENST00000260113

Gene Information

This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines and thus may play a role in the central nervous system. [provided by RefSeq Jul 2008]

Gene Symbol:   PI15
Gene Name:   peptidase inhibitor 15
Aliases:   CRISP8, DKFZp686F0366, P24TI, P25TI
RefSeq:   NC_000008.10 NT_008183.19
Ensembl:   ENSG00000137558
Entrez:   51050
UniGene:   Hs.98558
Chromosome Mapping:   8q21.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.540000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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