This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030229
PrimePCR™ PreAmp for SYBR® Green Assay: VPS4B, Human
PrimePCR™ Template for SYBR® Green Assay: VPS4B, Human
The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities) and is the homolog of the yeast Vps4 protein. In humans two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments and are involved in intracellular protein trafficking similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.