This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030218
PrimePCR™ PreAmp for SYBR® Green Assay: CRYBB1, Human
PrimePCR™ Template for SYBR® Green Assay: CRYBB1, Human
Crystallins are separated into two classes: taxon-specific or enzyme and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development these crystallins are made and then retained throughout life making them extremely stable proteins. Mammalian lens crystallins are divided into alpha beta and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs a connecting peptide and N- and C-terminal extensions. Beta-crystallins the most heterogeneous differ by the presence of the C-terminal extension (present in the basic group none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene a beta basic group member undergoes extensive cleavage at its N-terminal extension during lens maturation. It is also a member of a gene cluster with beta-A4 beta-B2 and beta-B3. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.