This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030205
PrimePCR™ PreAmp for SYBR® Green Assay: CCBE1, Human
PrimePCR™ Template for SYBR® Green Assay: CCBE1, Human
This gene is thought to function in extracellular matrix remodeling and migration. It is predominantly expressed in the ovary but down regulated in ovarian cancer cell lines and primary carcinomas suggesting its role as a tumour suppressor. Mutations in this gene have been associated with Hennekam lymphangiectasia-lymphedema syndrome a generalized lymphatic dysplasia in humans. [provided by RefSeq Mar 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.