This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030199
PrimePCR™ PreAmp for SYBR® Green Assay: GMPS, Human
PrimePCR™ Template for SYBR® Green Assay: GMPS, Human
In the de novo synthesis of purine nucleotides IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway there are 2 enzymes involved in converting IMP to GMP namely IMP dehydrogenase (IMPD1) which catalyzes the oxidation of IMP to XMP and GMP synthetase which catalyzes the amination of XMP to GMP. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.