This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030178
PrimePCR™ PreAmp for SYBR® Green Assay: ADAT1, Human
PrimePCR™ Template for SYBR® Green Assay: ADAT1, Human
This gene is a member of the ADAR (adenosine deaminase acting on RNA) family. Using site-specific adenosine modification proteins encoded by these genes participate in the pre-mRNA editing of nuclear transcripts. The protein encoded by this gene tRNA-specific adenosine deaminase 1 is responsible for the deamination of adenosine 37 to inosine in eukaryotic tRNA. Alternatively spliced transcript variants have been described. [provided by RefSeq Jul 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.