This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030176
PrimePCR™ PreAmp for SYBR® Green Assay: GNRH1, Human
PrimePCR™ Template for SYBR® Green Assay: GNRH1, Human
The protein encoded by this gene is secreted and then cleaved to form the 10 aa luteinizing hormone-releasing hormone (LHRH) also known as gonadoliberin-1 and prolactin release-inhibiting factor also known as GnRH-associated peptide 1. LHRH stimulates the release of luteinizing and follicle stimulating hormones which are important for reproduction. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jan 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.