This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030100
PrimePCR™ PreAmp for SYBR® Green Assay: TERF2, Human
PrimePCR™ Template for SYBR® Green Assay: TERF2, Human
This gene encodes a telomere specific protein TERF2 which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization TERF2 differs from TERF1 in that its N terminus is basic rather than acidic. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.