This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030078
PrimePCR™ PreAmp for SYBR® Green Assay: SUCNR1, Human
PrimePCR™ Template for SYBR® Green Assay: SUCNR1, Human
This gene encodes a G-protein-coupled receptor for succinate an intermediate molecule of the citric acid cycle. It is involved in the promotion of hematopoietic progenitor cell development and it has a potential role in renovascular hypertension which has known correlations to renal failure diabetes and atherosclerosis. [provided by RefSeq Oct 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.