This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030021
PrimePCR™ PreAmp for SYBR® Green Assay: SEPT14, Human
PrimePCR™ Template for SYBR® Green Assay: SEPT14, Human
SEPT14 is a member of the highly conserved septin family of GTP-binding cytoskeletal proteins implicated in membrane transport apoptosis cell polarity cell cycle regulation cytokinesis and other cellular functions (Peterson et al. 2007 [PubMed 17922164]).[supplied by OMIM Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.