This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030013
PrimePCR™ PreAmp for SYBR® Green Assay: PRL, Human
PrimePCR™ Template for SYBR® Green Assay: PRL, Human
This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.