PrimePCR™ SYBR® Green Assay: CNGA1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015540
Assay Design:   Intron-spanning
Chromosome Location:   4:47953440-47954679question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000402813 ENST00000514170 ENST00000420489 ENST00000504722 ENST00000514520 ENST00000513178 ENST00000544810 ENST00000358519

Gene Information

The protein encoded by this gene is involved in phototransduction. Along with another protein the encoded protein forms a cGMP-gated cation channel in the plasma membrane allowing depolarization of rod photoreceptors. This represents the last step in the phototransduction pathway. Defects in this gene are a cause of retinitis pigmentosa autosomal recessive (ARRP) disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2008]

Gene Symbol:   CNGA1
Gene Name:   cyclic nucleotide gated channel alpha 1
Aliases:   CNCG, CNCG1, CNG1, RCNC1, RCNCa, RCNCalpha, RP49
RefSeq:   NC_000004.11 NT_006238.11 NG_009193.1
Ensembl:   ENSG00000198515
Entrez:   1259
UniGene:   Hs.1323
Chromosome Mapping:   4p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.930000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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