PrimePCR™ SYBR® Green Assay: ST8SIA5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030001

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ST8SIA5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ST8SIA5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015533
Assay Design:   Intron-spanning
Chromosome Location:   18:44266208-44268798question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000315087 ENST00000538168 ENST00000536490

Gene Information

The protein encoded by this gene is a type II membrane protein that may be present in the Golgi apparatus. The encoded protein which is a member of glycosyltransferase family 29 may be involved in the synthesis of gangliosides GD1c GT1a GQ1b and GT3 from GD1a GT1b GM1b and GD3 respectively. [provided by RefSeq Jul 2008]

Gene Symbol:   ST8SIA5
Gene Name:   ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5
Aliases:   MGC119670, MGC119671, SIAT8E, ST8SiaV
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000101638
Entrez:   29906
Chromosome Mapping:   18q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.030000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download