This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029972
PrimePCR™ PreAmp for SYBR® Green Assay: FRMPD4, Human
PrimePCR™ Template for SYBR® Green Assay: FRMPD4, Human
This gene encodes a multi-domain (WW PDZ FERM) containing protein. Through its interaction with other proteins (such as PSD-95) it functions as a positive regulator of dendritic spine morphogenesis and density and is required for the maintenance of excitatory synaptic transmission. [provided by RefSeq Jan 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.