PrimePCR™ SYBR® Green Assay: FRMPD4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029972

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FRMPD4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FRMPD4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015487
Assay Design:   Intron-spanning
Chromosome Location:   X:12728575-12734275question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000380682 ENST00000429478 ENST00000304087

Gene Information

This gene encodes a multi-domain (WW PDZ FERM) containing protein. Through its interaction with other proteins (such as PSD-95) it functions as a positive regulator of dendritic spine morphogenesis and density and is required for the maintenance of excitatory synaptic transmission. [provided by RefSeq Jan 2010]

Gene Symbol:   FRMPD4
Gene Name:   FERM and PDZ domain containing 4
Aliases:   KIAA0316, MGC142260, MGC142262, PDZD10, PDZK10
RefSeq:   NC_000023.10 NG_016419.1 NT_167197.1
Ensembl:   ENSG00000169933
Entrez:   9758
Chromosome Mapping:   Xp22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.370000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download