PrimePCR™ SYBR® Green Assay: AMTN, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015410
Assay Design:   Intron-spanning
Chromosome Location:   4:71396980-71398233question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000339336 ENST00000504451

Gene Information

The mineralized portions of teeth the dentin and enamel are formed by mesenchyme-derived odontoblasts and epithelium-derived ameloblasts respectively. As ameloblasts differentiate they deposit specific proteins necessary for enamel formation including amelogenin (AMELX; MIM 300391) enamelin (ENAM; MIM 606585) and ameloblastin (AMBN; MIM 601259) in the organic enamel matrix. Amelotin is specifically expressed in maturation-stage ameloblasts (Iwasaki et al. 2005 [PubMed 16304441]).[supplied by OMIM Mar 2008]

Gene Symbol:   AMTN
Gene Name:   amelotin
Aliases:   MGC148132, MGC148133, UNQ689
RefSeq:   NC_000004.11 NT_022778.16
Ensembl:   ENSG00000187689
Entrez:   401138
UniGene:   Hs.453069
Chromosome Mapping:   4q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.780000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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